منابع مشابه
DNA microarrays, chromatin, transcription
The DNA microarray-based technique has been developed to semi-quantitatively measure the in vivo global chromatin condensation state at the resolution of a gene. Chromatin was fractionated due to the differential solubility of histone H1-containing and histone H1-free nucleosomes. A set of genes non-randomly distributed between histone H1-free (uncondensed or open) and histone H1-containing (co...
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The question of how sequence-specific transcription factors access their cognate sites in nucleosomally organized DNA is discussed on the basis of genomic footprinting data and chromatin reconstitution experiments. A classification of factors into two categories is proposed: (i) initiator factors which are able to bind their target sequences within regular nucleosomes and initiate events leadin...
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Tousled-like kinases (TLKs) have important functions during the cell cycle in eukaryotes. The TLK family was named after its founding member, the product of the TOUSLED gene which is essential for normal development of the plant Arabidopsis thaliana [1]. More recently, these kinases have been implicated in deposition of chromatin following DNA replication. During S phase, nucleosomes must be as...
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A factor has been purified that specifically facilitates RNA chain elongation in a chromatin context; the properties of this factor help to define the challenges that lie ahead in understanding how RNA polymerase manages to transcribe DNA that is packaged into chromatin.
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As outlined in the preceding chapters, an outstanding problem in the field of eukaryotic gene regulation is understanding the biochemical mechanism by which gene-specific activators regulate transcription. In Chapters 11 and 12, we discussed biochemical strategies to dissect how these transcriptional regulators interact with their cognate DNA sequences and enhance transcription from DNA templat...
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ژورنال
عنوان ژورنال: Nature
سال: 1977
ISSN: 0028-0836,1476-4687
DOI: 10.1038/266304a0